Differential miRNA expression in primary tumors and metastasis

The Medical Oncology department and TGAC have teamed up to investigate differential micro RNA expression in primary tumors, metastasis and normal tissue. This project, led by Maarten Neerincx and Daoud Sie, processed 220 samples and subjected them to miRNA expression profiling through NGS on a HiSeq 2500. An average of 10 millions reads per samples has been obtained by generating over 2 billion reads and 100 GB of data in total. The data has been processed with mirdeep to quantify miRNA expression and to detect novel miRNA candidates. The results of the project have been published in Nature Oncogensis.

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